Part:BBa_K783067:Design

pBad-pTetR inverter

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1247
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1187
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 979
  Illegal AgeI site found at 3490
  Illegal AgeI site found at 3602
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2634
  Illegal SapI site found at 961
  

Design Notes

We changed the RBS strength driving the tetR gene and this yielded better results.

Source

The source of this device is from the BioBrick parts used to build it.